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TitlePotent and selective inhibition of pathogenic viruses by engineered ubiquitin variants.
Journal, issue, pagesPLoS Pathog., Vol. 13, Page e1006372-e1006372, Year 2017
Publish dateMar 14, 2017 (structure data deposition date)
AuthorsZhang, W. / Bailey-Elkin, B.A. / Knaap, R.C.M. / Khare, B. / Dalebout, T.J. / Johnson, G.G. / van Kasteren, P.B. / McLeish, N.J. / Gu, J. / He, W. ...Zhang, W. / Bailey-Elkin, B.A. / Knaap, R.C.M. / Khare, B. / Dalebout, T.J. / Johnson, G.G. / van Kasteren, P.B. / McLeish, N.J. / Gu, J. / He, W. / Kikkert, M. / Mark, B.L. / Sidhu, S.S.
External linksPLoS Pathog. / PubMed:28542609
MethodsX-ray diffraction
Resolution1.5 - 2.7 Å
Structure data

PDB-5v5g:
OTU protease of Crimean Congo Hemorrhagic Fever Virus bound to ubiquitin variant CC.4
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-5v5h:
OTU protease of Crimean Congo Hemorrhagic Fever Virus bound to ubiquitin variant CC.2
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-5v69:
Crystal structure of the Middle East respiratory syndrome coronavirus papain-like protease bound to ubiquitin variant ME.4
Method: X-RAY DIFFRACTION / Resolution: 2.55 Å

PDB-5v6a:
Crystal structure of the Middle East respiratory syndrome coronavirus papain-like protease bound to ubiquitin variant ME.2
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

Chemicals

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-NA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-ZN:
Unknown entry

ChemComp-EPE:
4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / pH buffer*YM

ChemComp-CL:
Unknown entry

ChemComp-PGO:
S-1,2-PROPANEDIOL

ChemComp-FLC:
CITRATE ANION

Source
  • crimean-congo hemorrhagic fever virus (strain nigeria/ibar10200/1970)
  • synthetic construct (others)
  • human betacoronavirus 2c emc/2012
  • homo sapiens (human)
Keywordshydrolase / transferase / Ovarian Tumor Domain protease / deubiquitinase / ubiquitin variant / PLpro / ubiquitin / DUB

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