+検索条件
-Structure paper
タイトル | Immunization-Elicited Broadly Protective Antibody Reveals Ebolavirus Fusion Loop as a Site of Vulnerability. |
---|---|
ジャーナル・号・ページ | Cell, Vol. 169, Issue 5, Page 891-904.e15, Year 2017 |
掲載日 | 2017年5月18日 |
![]() | Xuelian Zhao / Katie A Howell / Shihua He / Jennifer M Brannan / Anna Z Wec / Edgar Davidson / Hannah L Turner / Chi-I Chiang / Lin Lei / J Maximilian Fels / Hong Vu / Sergey Shulenin / Ashley N Turonis / Ana I Kuehne / Guodong Liu / Mi Ta / Yimeng Wang / Christopher Sundling / Yongli Xiao / Jennifer S Spence / Benjamin J Doranz / Frederick W Holtsberg / Andrew B Ward / Kartik Chandran / John M Dye / Xiangguo Qiu / Yuxing Li / M Javad Aman / ![]() ![]() ![]() |
PubMed 要旨 | While neutralizing antibodies are highly effective against ebolavirus infections, current experimental ebolavirus vaccines primarily elicit species-specific antibody responses. Here, we describe an ...While neutralizing antibodies are highly effective against ebolavirus infections, current experimental ebolavirus vaccines primarily elicit species-specific antibody responses. Here, we describe an immunization-elicited macaque antibody (CA45) that clamps the internal fusion loop with the N terminus of the ebolavirus glycoproteins (GPs) and potently neutralizes Ebola, Sudan, Bundibugyo, and Reston viruses. CA45, alone or in combination with an antibody that blocks receptor binding, provided full protection against all pathogenic ebolaviruses in mice, guinea pigs, and ferrets. Analysis of memory B cells from the immunized macaque suggests that elicitation of broadly neutralizing antibodies (bNAbs) for ebolaviruses is possible but difficult, potentially due to the rarity of bNAb clones and their precursors. Unexpectedly, germline-reverted CA45, while exhibiting negligible binding to full-length GP, bound a proteolytically remodeled GP with picomolar affinity, suggesting that engineered ebolavirus vaccines could trigger rare bNAb precursors more robustly. These findings have important implications for developing pan-ebolavirus vaccine and immunotherapeutic cocktails. |
![]() | ![]() ![]() ![]() |
手法 | EM (単粒子) |
解像度 | 11.0 Å |
構造データ | ![]() EMDB-8694: |
由来 |
|