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Structure paper

TitleAssessing the Influence of Mutation on GTPase Transition States by Using X-ray Crystallography, (19) F NMR, and DFT Approaches.
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 56, Page 9732-9735, Year 2017
Publish dateOct 26, 2016 (structure data deposition date)
AuthorsJin, Y. / Molt, R.W. / Pellegrini, E. / Cliff, M.J. / Bowler, M.W. / Richards, N.G.J. / Blackburn, G.M. / Waltho, J.P.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:28498638
MethodsX-ray diffraction
Resolution2.2 - 2.4 Å
Structure data

PDB-5m6x:
Crystal Structure of human RhoGAP mutated in its arginine finger (R85A) in complex with RhoA.GDP.MgF3- human
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-5m70:
Crystal Structure of human RhoGAP mutated in its arginin finger (R85A) in complex with RhoA.GDP.AlF4- human
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-MGF:
TRIFLUOROMAGNESATE

ChemComp-HOH:
WATER

ChemComp-ALF:
TETRAFLUOROALUMINATE ION

Source
  • homo sapiens (human)
KeywordsSIGNALING PROTEIN / RHOGAP / Arginine finger / RHOA / transition state

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