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TitleStructural investigations on orotate phosphoribosyltransferase from Mycobacterium tuberculosis, a key enzyme of the de novo pyrimidine biosynthesis.
Journal, issue, pagesSci Rep, Vol. 7, Page 1180-1180, Year 2017
Publish dateJan 14, 2016 (structure data deposition date)
AuthorsDonini, S. / Ferraris, D.M. / Miggiano, R. / Massarotti, A. / Rizzi, M.
External linksSci Rep / PubMed:28446777
MethodsX-ray diffraction
Resolution1.899 - 2.4 Å
Structure data

PDB-5hkf:
Crystal structure of Mycobacterium tuberculosis H37Rv orotate phosphoribosyltransferase in complex with 5-phospho-alpha-D-ribosyl 1-diphosphate (PRPP)
Method: X-RAY DIFFRACTION / Resolution: 2.25 Å

PDB-5hki:
Crystal structure of Mycobacterium tuberculosis H37Rv orotate phosphoribosyltransferase in complex with Fe(III) dicitrate
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-5hkl:
Crystal structure of Mycobacterium tuberculosis H37Rv orotate phosphoribosyltransferase in complex with inorganic phosphate
Method: X-RAY DIFFRACTION / Resolution: 1.899 Å

Chemicals

ChemComp-PRP:
1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose

ChemComp-HOH:
WATER

ChemComp-6ZJ:
Iron(III) dicitrate

ChemComp-PO4:
PHOSPHATE ION

Source
  • mycobacterium tuberculosis (strain atcc 25618 / h37rv) (bacteria)
KeywordsTRANSFERASE / OPRT / de novo pyrimidine nucleotide synthesis / PRPP complex / ferric dicitrate complex / inorganic phosphate complex

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