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Structure paper

TitleStructure-Based Engineering of an Artificially Generated NADP+-Dependent d-Amino Acid Dehydrogenase
Journal, issue, pagesAppl. Environ. Microbiol., Vol. 83, Year 2017
Publish dateSep 26, 2016 (structure data deposition date)
AuthorsHayashi, J. / Seto, T. / Akita, H. / Watanabe, M. / Hoshino, T. / Yoneda, K. / Ohshima, T. / Sakuraba, H.
External linksAppl. Environ. Microbiol. / PubMed:28363957
MethodsX-ray diffraction
Resolution1.59 - 1.78 Å
Structure data

PDB-5gz1:
Structure of substrate/cofactor-free D-amino acid dehydrogenase
Method: X-RAY DIFFRACTION / Resolution: 1.78 Å

PDB-5gz3:
Structure of D-amino acid dehydrogenase in complex with NADP
Method: X-RAY DIFFRACTION / Resolution: 1.59 Å

PDB-5gz6:
Structure of D-amino acid dehydrogenase in complex with NADPH and 2-keto-6-aminocapronic acid
Method: X-RAY DIFFRACTION / Resolution: 1.74 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-7C3:
6-azanyl-2-oxidanylidene-hexanoic acid

ChemComp-SO4:
SULFATE ION

ChemComp-ACT:
ACETATE ION

Source
  • ureibacillus thermosphaericus (bacteria)
KeywordsOXIDOREDUCTASE / Rossmann fold

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