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TitleIn Situ Molecular Architecture of the Salmonella Type III Secretion Machine.
Journal, issue, pagesCell, Vol. 168, Issue 6, Page 1065-1074.e10, Year 2017
Publish dateMar 9, 2017
AuthorsBo Hu / Maria Lara-Tejero / Qingke Kong / Jorge E Galán / Jun Liu /
PubMed AbstractType III protein secretion systems have specifically evolved to deliver bacterially encoded proteins into target eukaryotic cells. The core elements of this multi-protein machine are the envelope- ...Type III protein secretion systems have specifically evolved to deliver bacterially encoded proteins into target eukaryotic cells. The core elements of this multi-protein machine are the envelope-associated needle complex, the inner membrane export apparatus, and a large cytoplasmic sorting platform. Here, we report a high-resolution in situ structure of the Salmonella Typhimurium type III secretion machine obtained by high-throughput cryo-electron tomography and sub-tomogram averaging. Through molecular modeling and comparative analysis of machines assembled with protein-tagged components or from different deletion mutants, we determined the molecular architecture of the secretion machine in situ and localized its structural components. We also show that docking of the sorting platform results in significant conformational changes in the needle complex to provide the symmetry adaptation required for the assembly of the entire secretion machine. These studies provide major insight into the structure and assembly of a broadly distributed protein secretion machine.
External linksCell / PubMed:28283062 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution17.0 - 30.0 Å
Structure data

EMDB-8544:
Salmonella Type III secretion injectisome
Method: EM (subtomogram averaging) / Resolution: 17.0 Å

EMDB-8545:
SpaO mutant structure of Salmonella Type III secretion injectisome
Method: EM (subtomogram averaging) / Resolution: 30.0 Å

Source
  • Salmonella (bacteria)

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