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TitleThe dynamic assembly of distinct RNA polymerase I complexes modulates rDNA transcription.
Journal, issue, pagesElife, Vol. 6, Year 2017
Publish dateMar 6, 2017
AuthorsEva Torreira / Jaime Alegrio Louro / Irene Pazos / Noelia González-Polo / David Gil-Carton / Ana Garcia Duran / Sébastien Tosi / Oriol Gallego / Olga Calvo / Carlos Fernández-Tornero /
PubMed AbstractCell growth requires synthesis of ribosomal RNA by RNA polymerase I (Pol I). Binding of initiation factor Rrn3 activates Pol I, fostering recruitment to ribosomal DNA promoters. This fundamental ...Cell growth requires synthesis of ribosomal RNA by RNA polymerase I (Pol I). Binding of initiation factor Rrn3 activates Pol I, fostering recruitment to ribosomal DNA promoters. This fundamental process must be precisely regulated to satisfy cell needs at any time. We present in vivo evidence that, when growth is arrested by nutrient deprivation, cells induce rapid clearance of Pol I-Rrn3 complexes, followed by the assembly of inactive Pol I homodimers. This dual repressive mechanism reverts upon nutrient addition, thus restoring cell growth. Moreover, Pol I dimers also form after inhibition of either ribosome biogenesis or protein synthesis. Our mutational analysis, based on the electron cryomicroscopy structures of monomeric Pol I alone and in complex with Rrn3, underscores the central role of subunits A43 and A14 in the regulation of differential Pol I complexes assembly and subsequent promoter association.
External linksElife / PubMed:28262097 / PubMed Central
MethodsEM (single particle)
Resolution4.9 - 7.7 Å
Structure data

EMDB-4086:
RNA polymerase I-Rrn3 complex
Method: EM (single particle) / Resolution: 7.7 Å

EMDB-4087:
Monomeric RNA polymerase I at 5.6 A resolution
Method: EM (single particle) / Resolution: 5.6 Å

4088

5lmx

EMDB-4088, PDB-5lmx:
Monomeric RNA polymerase I at 4.9 A resolution
Method: EM (single particle) / Resolution: 4.9 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • Saccharomyces cerevisiae (brewer's yeast)
  • Baker's yeast (brewer's yeast)
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
KeywordsTRANSCRIPTION / Multi-protein complex / RNA polymerase / Monomeric form

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