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TitleDissecting the molecular organization of the translocon-associated protein complex.
Journal, issue, pagesNat Commun, Vol. 8, Page 14516, Year 2017
Publish dateFeb 20, 2017
AuthorsStefan Pfeffer / Johanna Dudek / Miroslava Schaffer / Bobby G Ng / Sahradha Albert / Jürgen M Plitzko / Wolfgang Baumeister / Richard Zimmermann / Hudson H Freeze / Benjamin D Engel / Friedrich Förster /
PubMed AbstractIn eukaryotic cells, one-third of all proteins must be transported across or inserted into the endoplasmic reticulum (ER) membrane by the ER protein translocon. The translocon-associated protein ...In eukaryotic cells, one-third of all proteins must be transported across or inserted into the endoplasmic reticulum (ER) membrane by the ER protein translocon. The translocon-associated protein (TRAP) complex is an integral component of the translocon, assisting the Sec61 protein-conducting channel by regulating signal sequence and transmembrane helix insertion in a substrate-dependent manner. Here we use cryo-electron tomography (CET) to study the structure of the native translocon in evolutionarily divergent organisms and disease-linked TRAP mutant fibroblasts from human patients. The structural differences detected by subtomogram analysis form a basis for dissecting the molecular organization of the TRAP complex. We assign positions to the four TRAP subunits within the complex, providing insights into their individual functions. The revealed molecular architecture of a central translocon component advances our understanding of membrane protein biogenesis and sheds light on the role of TRAP in human congenital disorders of glycosylation.
External linksNat Commun / PubMed:28218252 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution14.9 - 21.7 Å
Structure data

EMDB-4143:
Subtomogram average of the ER membrane-associated ribosome from human TRAPdelta-deficient fibroblasts
Method: EM (subtomogram averaging) / Resolution: 14.9 Å

EMDB-4144:
Subtomogram average of the ER membrane-associated ribosome from human TRAPgamma-deficient fibroblasts
Method: EM (subtomogram averaging) / Resolution: 21.7 Å

EMDB-4145:
Subtomogram average of the ER membrane-associated ribosome from FIB-milled C. reinhardtii cells
Method: EM (subtomogram averaging) / Resolution: 19.4 Å

Source
  • Homo sapiens (human)
  • Chlamydomonas reinhardtii (plant)

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