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Structure paper

TitleStructural Basis of Microtubule Stabilization by Discodermolide.
Journal, issue, pagesChembiochem, Vol. 18, Page 905-909, Year 2017
Publish dateSep 22, 2016 (structure data deposition date)
AuthorsProta, A.E. / Bargsten, K. / Redondo-Horcajo, M. / Smith, A.B. / Yang, C.H. / McDaid, H.M. / Paterson, I. / Horwitz, S.B. / Fernando Diaz, J. / Steinmetz, M.O.
External linksChembiochem / PubMed:28207984
MethodsX-ray diffraction
Resolution1.9 - 2.2 Å
Structure data

PDB-5lxs:
Tubulin-KS-1-199-32 complex
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-5lxt:
Tubulin-Discodermolide complex
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-CA:
Unknown entry

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-7AO:
[(3~{Z},5~{S},6~{S},7~{S},8~{R},9~{S},11~{Z},13~{S},14~{S},15~{S},16~{Z},18~{S})-19-[(2~{S},3~{R},4~{S},5~{R})-3,5-dimethyl-4-oxidanyl-6-oxidanylidene-oxan-2-yl]-5,7,9,11,13,15-hexamethyl-8,14,18-tris(oxidanyl)nonadeca-1,3,11,16-tetraen-6-yl] ~{N}-[3-[(3-azidophenyl)carbonylamino]propyl]carbamate

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-ACP:
PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / AMP-PCP, energy-carrying molecule analogue*YM

ChemComp-HOH:
WATER

ChemComp-7AK:
(+)-Discodermolide

Source
  • rattus norvegicus (Norway rat)
  • gallus gallus (chicken)
  • bos taurus (domestic cattle)
KeywordsCELL CYCLE / TUBULIN FOLD / CYTOSKELETON / MICROTUBULE / DISCODERMOLIDE

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