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Structure paper

TitleExtending enzyme molecular recognition with an expanded amino acid alphabet.
Journal, issue, pagesProc. Natl. Acad. Sci. U.S.A., Vol. 114, Page 2610-2615, Year 2017
Publish dateJul 25, 2016 (structure data deposition date)
AuthorsWindle, C.L. / Simmons, K.J. / Ault, J.R. / Trinh, C.H. / Nelson, A. / Pearson, A.R. / Berry, A.
External linksProc. Natl. Acad. Sci. U.S.A. / PubMed:28196894
MethodsX-ray diffraction
Resolution1.7 Å
Structure data

PDB-5lky:
X-ray crystal structure of N-acetylneuraminic acid lyase in complex with pyruvate, with the phenylalanine at position 190 replaced with the non-canonical amino acid dihydroxypropylcysteine.
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

Chemicals

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-HOH:
WATER

Source
  • staphylococcus aureus (strain nctc 8325) (bacteria)
KeywordsLYASE / aldolase / non-canonical amino acid / TIM barrel

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