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Structure paper

TitleHydroxyl regioisomerization of anthracycline catalyzed by a four-enzyme cascade
Journal, issue, pagesProc. Natl. Acad. Sci. U.S.A., Vol. 114, Page 1554-1559, Year 2017
Publish dateDec 4, 2015 (structure data deposition date)
AuthorsZhang, Z. / Gong, Y.-K. / Zhou, Q. / Hu, Y. / Ma, H.-M. / Chen, Y.-S. / Igarashi, Y. / Pan, L. / Tang, G.-L.
External linksProc. Natl. Acad. Sci. U.S.A. / PubMed:28137838
MethodsX-ray diffraction
Resolution1.304 - 1.68 Å
Structure data

PDB-5f5l:
The structure of monooxygenase KstA11 in the biosynthetic pathway of kosinostatin
Method: X-RAY DIFFRACTION / Resolution: 1.68 Å

PDB-5f5n:
The structure of monooxygenase KstA11 in complex with NAD and its substrate
Method: X-RAY DIFFRACTION / Resolution: 1.304 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-DTT:
2,3-DIHYDROXY-1,4-DITHIOBUTANE

ChemComp-HOH:
WATER

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

ChemComp-5VD:
methyl (1~{R},2~{R},4~{S})-2-methyl-2,4,5,7,10-pentakis(oxidanyl)-6,11-bis(oxidanylidene)-3,4-dihydro-1~{H}-tetracene-1-carboxylate

ChemComp-EPE:
4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / pH buffer*YM

ChemComp-MG:
Unknown entry

Source
  • micromonospora sp. tp-a0468 (bacteria)
KeywordsOXIDOREDUCTASE / monooxygenase / dearomatization / kosinostatin / complex

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