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TitleUsing the Volta phase plate with defocus for cryo-EM single particle analysis.
Journal, issue, pagesElife, Vol. 6, Year 2017
Publish dateJan 21, 2017
AuthorsRadostin Danev / Dimitry Tegunov / Wolfgang Baumeister /
PubMed AbstractPreviously, we reported an in-focus data acquisition method for cryo-EM single-particle analysis with the Volta phase plate (Danev and Baumeister, 2016). Here, we extend the technique to include a ...Previously, we reported an in-focus data acquisition method for cryo-EM single-particle analysis with the Volta phase plate (Danev and Baumeister, 2016). Here, we extend the technique to include a small amount of defocus which enables contrast transfer function measurement and correction. This hybrid approach simplifies the experiment and increases the data acquisition speed. It also removes the resolution limit inherent to the in-focus method thus allowing 3D reconstructions with resolutions better than 3 Å.
External linksElife / PubMed:28109158 / PubMed Central
MethodsEM (single particle)
Resolution2.2 - 2.4 Å
Structure data

EMDB-3455:
Thermoplasma acidophilum 20S proteasome map reconstructed with Relion 2.0 particle polishing from defocused Volta phase plate cryo-EM data
Method: EM (single particle) / Resolution: 2.4 Å

EMDB-3456:
Thermoplasma acidophilum 20S proteasome map reconstructed with MotionCor2 and Relion 2.0 from defocused Volta phase plate cryo-EM data
Method: EM (single particle) / Resolution: 2.4 Å

EMDB-3457:
Thermoplasma acidophilum 20S proteasome map reconstructed with Warp and Relion 2.0 from defocused Volta phase plate cryo-EM data
Method: EM (single particle) / Resolution: 2.2 Å

Source
  • Thermoplasma acidophilum (acidophilic)

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