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Structure paper

TitleStructural insights into the mechanism of the DEAH-box RNA helicase Prp43.
Journal, issue, pagesElife, Vol. 6, Year 2017
Publish dateSep 6, 2016 (structure data deposition date)
AuthorsTauchert, M.J. / Fourmann, J.B. / Luhrmann, R. / Ficner, R.
External linksElife / PubMed:28092261
MethodsX-ray diffraction
Resolution1.78 - 3.241 Å
Structure data

PDB-5lta:
Crystal structure of the Prp43-ADP-BeF3-U7-RNA complex
Method: X-RAY DIFFRACTION / Resolution: 2.621 Å

PDB-5ltj:
Crystal structure of the Prp43-ADP-BeF3 complex (in orthorhombic space group)
Method: X-RAY DIFFRACTION / Resolution: 1.78 Å

PDB-5ltk:
Crystal structure of the Prp43-ADP-BeF3 complex (in hexagonal space group)
Method: X-RAY DIFFRACTION / Resolution: 3.241 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-BEF:
BERYLLIUM TRIFLUORIDE ION

ChemComp-CAC:
CACODYLATE ION

ChemComp-HOH:
WATER

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-MRD:
(4R)-2-METHYLPENTANE-2,4-DIOL / precipitant*YM

ChemComp-SO4:
SULFATE ION

ChemComp-CL:
Unknown entry

Source
  • chaetomium thermophilum var. thermophilum dsm 1495 (fungus)
  • synthetic construct (others)
KeywordsProtein/RNA / RNA HELICASE / DEAH-BOX PROTEIN / DHX15 / Protein-RNA complex / HYDROLASE / Spliceosome

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