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TitleNew insights into the GINS complex explain the controversy between existing structural models.
Journal, issue, pagesSci Rep, Vol. 7, Page 40188, Year 2017
Publish dateJan 10, 2017
AuthorsMarta Carroni / Matteo De March / Barbara Medagli / Ivet Krastanova / Ian A Taylor / Heinz Amenitsch / Hiroyuchi Araki / Francesca M Pisani / Ardan Patwardhan / Silvia Onesti /
PubMed AbstractGINS is a key component of eukaryotic replicative forks and is composed of four subunits (Sld5, Psf1, Psf2, Psf3). To explain the discrepancy between structural data from crystallography and electron ...GINS is a key component of eukaryotic replicative forks and is composed of four subunits (Sld5, Psf1, Psf2, Psf3). To explain the discrepancy between structural data from crystallography and electron microscopy (EM), we show that GINS is a compact tetramer in solution as observed in crystal structures, but also forms a double-tetrameric population, detectable by EM. This may represent an intermediate step towards the assembly of two replicative helicase complexes at origins, moving in opposite directions within the replication bubble. Reconstruction of the double-tetrameric form, combined with small-angle X-ray scattering data, allows the localisation of the B domain of the Psf1 subunit in the free GINS complex, which was not visible in previous studies and is essential for the formation of a functional replication fork.
External linksSci Rep / PubMed:28071757 / PubMed Central
MethodsEM (single particle)
Resolution30.0 Å
Structure data

EMDB-2640:
Negative-stain electron microscopy of the human GINS complex
Method: EM (single particle) / Resolution: 30.0 Å

Source
  • Homo sapiens (human)

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