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Structure paper

TitleA Single Amino Acid Mutation Converts (R)-5-Diphosphomevalonate Decarboxylase into a Kinase
Journal, issue, pagesJ. Biol. Chem., Vol. 292, Page 2457-2469, Year 2017
Publish dateJul 13, 2016 (structure data deposition date)
AuthorsMotoyama, K. / Unno, H. / Hattori, A. / Takaoka, T. / Ishikita, H. / Kawaide, H. / Yoshimura, T. / Hemmi, H.
External linksJ. Biol. Chem. / PubMed:28003359
MethodsX-ray diffraction
Resolution1.5 - 1.7 Å
Structure data

PDB-5gmd:
Crystal structure of Sulfolobus solfataricus diphosphomevalonate decarboxylase in complex with ATP-gamma-S
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-5gme:
Crystal structure of Sulfolobus solfataricus Diphosphomevalonate decarboxylase in complex with ADP
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

Chemicals

ChemComp-DP6:
(3R)-3-HYDROXY-5-{[(R)-HYDROXY(PHOSPHONOOXY)PHOSPHORYL]OXY}-3-METHYLPENTANOIC ACID

ChemComp-AGS:
PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-gamma-S, energy-carrying molecule analogue*YM

ChemComp-AMP:
ADENOSINE MONOPHOSPHATE / AMP*YM

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

Source
  • sulfolobus solfataricus (strain atcc 35092 / dsm 1617 / jcm 11322 / p2) (archaea)
KeywordsLYASE / Sulfolobus solfataricus / diphosphomevalonate decarboxylase / ATPrS / ADP

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