Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	The structure and flexibility of conical HIV-1 capsids determined within intact virions.
Journal, issue, pages	Science, Vol. 354, Issue 6318, Page 1434-1437, Year 2016
Publish date	Dec 16, 2016
Authors	Simone Mattei / Bärbel Glass / Wim J H Hagen / Hans-Georg Kräusslich / John A G Briggs /
PubMed Abstract	HIV-1 contains a cone-shaped capsid encasing the viral genome. This capsid is thought to follow fullerene geometry-a curved hexameric lattice of the capsid protein, CA, closed by incorporating 12 CA ...HIV-1 contains a cone-shaped capsid encasing the viral genome. This capsid is thought to follow fullerene geometry-a curved hexameric lattice of the capsid protein, CA, closed by incorporating 12 CA pentamers. Current models for core structure are based on crystallography of hexameric and cross-linked pentameric CA, electron microscopy of tubular CA arrays, and simulations. Here, we report subnanometer-resolution cryo-electron tomography structures of hexameric and pentameric CA within intact HIV-1 particles. Whereas the hexamer structure is compatible with crystallography studies, the pentamer forms using different interfaces. Determining multiple structures revealed how CA flexes to form the variably curved core shell. We show that HIV-1 CA assembles both aberrant and perfect fullerene cones, supporting models in which conical cores assemble de novo after maturation.
External links	Science / PubMed:27980210
Methods	EM (tomography) / EM (subtomogram averaging)
Resolution	6.8 - 8.8 Å
Structure data	EMDB-3464: Cryo-electron tomogram containing mature HIV-1 particles Method: EM (tomography) 3465 5mcx EMDB-3465, PDB-5mcx: The structure of the mature HIV-1 CA hexamer in intact virus particles Method: EM (subtomogram averaging) / Resolution: 6.8 Å 3466 5mcy EMDB-3466, PDB-5mcy: The structure of the mature HIV-1 CA pentamer in intact virus particles Method: EM (subtomogram averaging) / Resolution: 8.8 Å 3467 5mcz EMDB-3467, PDB-5mcz: The structure of the mature HIV-1 CA hexameric lattice with curvature parameters: tilt=-1, twist=0 Method: EM (subtomogram averaging) / Resolution: 8.2 Å 3468 5md0 EMDB-3468, PDB-5md0: The structure of the mature HIV-1 CA hexameric lattice with curvature parameters: tilt=5, twist=6 Method: EM (subtomogram averaging) / Resolution: 8.4 Å 3469 5md1 EMDB-3469, PDB-5md1: The structure of the mature HIV-1 CA hexameric lattice with curvature parameters: tilt=5, twist=0 Method: EM (subtomogram averaging) / Resolution: 8.2 Å 3470 5md2 EMDB-3470, PDB-5md2: The structure of the mature HIV-1 CA hexameric lattice with curvature parameters: tilt=5, twist=-6 Method: EM (subtomogram averaging) / Resolution: 8.6 Å 3471 5md3 EMDB-3471, PDB-5md3: The structure of the mature HIV-1 CA hexameric lattice with curvature parameters: tilt=11, twist=12 Method: EM (subtomogram averaging) / Resolution: 8.5 Å 3472 5md4 EMDB-3472, PDB-5md4: The structure of the mature HIV-1 CA hexameric lattice with curvature parameters: tilt=11, twist=6 Method: EM (subtomogram averaging) / Resolution: 8.4 Å 3473 5md5 EMDB-3473, PDB-5md5: The structure of the mature HIV-1 CA hexameric lattice with curvature parameters: tilt=11, twist=0 Method: EM (subtomogram averaging) / Resolution: 8.2 Å 3474 5md6 EMDB-3474, PDB-5md6: The structure of the mature HIV-1 CA hexameric lattice with curvature parameters: tilt=11, twist=-6 Method: EM (subtomogram averaging) / Resolution: 8.1 Å 3475 5md7 EMDB-3475, PDB-5md7: The structure of the mature HIV-1 CA hexameric lattice with curvature parameters: tilt=11, twist=-12 Method: EM (subtomogram averaging) / Resolution: 8.4 Å 3477 5md8 EMDB-3477, PDB-5md8: The structure of the mature HIV-1 CA hexameric lattice with curvature parameters: tilt=17, twist=12 Method: EM (subtomogram averaging) / Resolution: 8.6 Å 3478 5md9 EMDB-3478, PDB-5md9: The structure of the mature HIV-1 CA hexameric lattice with curvature parameters: tilt=17, twist=6 Method: EM (subtomogram averaging) / Resolution: 8.0 Å 3479 5mda EMDB-3479, PDB-5mda: The structure of the mature HIV-1 CA hexameric lattice with curvature parameters: tilt=17, twist=0 Method: EM (subtomogram averaging) / Resolution: 8.4 Å 3480 5mdb EMDB-3480, PDB-5mdb: The structure of the mature HIV-1 CA hexameric lattice with curvature parameters: tilt=17, twist=-6 Method: EM (subtomogram averaging) / Resolution: 8.4 Å 3481 5mdc EMDB-3481, PDB-5mdc: The structure of the mature HIV-1 CA hexameric lattice with curvature parameters: tilt=17, twist=-12 Method: EM (subtomogram averaging) / Resolution: 8.4 Å 3482 5mdd EMDB-3482, PDB-5mdd: The structure of the mature HIV-1 CA hexameric lattice with curvature parameters: tilt=23, twist=6 Method: EM (subtomogram averaging) / Resolution: 8.5 Å 3483 5mde EMDB-3483, PDB-5mde: The structure of the mature HIV-1 CA hexameric lattice with curvature parameters: tilt=23, twist=0 Method: EM (subtomogram averaging) / Resolution: 8.4 Å 3484 5mdf EMDB-3484, PDB-5mdf: The structure of the mature HIV-1 CA hexameric lattice with curvature parameters: tilt=23, twist=-6 Method: EM (subtomogram averaging) / Resolution: 8.5 Å 3485 5mdg EMDB-3485, PDB-5mdg: The structure of the mature HIV-1 CA hexameric lattice with curvature parameters: tilt=29, twist=0 Method: EM (subtomogram averaging) / Resolution: 8.7 Å
Source	human immunodeficiency virus 1
Keywords	VIRAL PROTEIN / retrovirus / HIV-1 / capsid / hexamer / pentamer / lattice curvature