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TitleTranslational termination without a stop codon.
Journal, issue, pagesScience, Vol. 354, Issue 6318, Page 1437-1440, Year 2016
Publish dateDec 16, 2016
AuthorsNathan R James / Alan Brown / Yuliya Gordiyenko / V Ramakrishnan /
PubMed AbstractRibosomes stall when they encounter the end of messenger RNA (mRNA) without an in-frame stop codon. In bacteria, these "nonstop" complexes can be rescued by alternative ribosome-rescue factor A (ArfA) ...Ribosomes stall when they encounter the end of messenger RNA (mRNA) without an in-frame stop codon. In bacteria, these "nonstop" complexes can be rescued by alternative ribosome-rescue factor A (ArfA). We used electron cryomicroscopy to determine structures of ArfA bound to the ribosome with 3'-truncated mRNA, at resolutions ranging from 3.0 to 3.4 angstroms. ArfA binds within the ribosomal mRNA channel and substitutes for the absent stop codon in the A site by specifically recruiting release factor 2 (RF2), initially in a compact preaccommodated state. A similar conformation of RF2 may occur on stop codons, suggesting a general mechanism for release-factor-mediated translational termination in which a conformational switch leads to peptide release only when the appropriate signal is present in the A site.
External linksScience / PubMed:27934701 / PubMed Central
MethodsEM (single particle)
Resolution2.97 - 3.35 Å
Structure data

3489

5mdv

EMDB-3489, PDB-5mdv:
Structure of ArfA and RF2 bound to the 70S ribosome (accommodated state)
Method: EM (single particle) / Resolution: 2.97 Å

3490

5mdw

EMDB-3490, PDB-5mdw:
Structure of ArfA(A18T) and RF2 bound to the 70S ribosome (pre-accommodated state)
Method: EM (single particle) / Resolution: 3.06 Å

3492

5mdy

EMDB-3492, PDB-5mdy:
Structure of ArfA and TtRF2 bound to the 70S ribosome (pre-accommodated state)
Method: EM (single particle) / Resolution: 3.35 Å

3493

5mdz

EMDB-3493, PDB-5mdz:
Structure of the 70S ribosome (empty A site)
Method: EM (single particle) / Resolution: 3.1 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-FME:
N-FORMYLMETHIONINE

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER

Source
  • escherichia coli (E. coli)
  • escherichia coli k-12 (bacteria)
  • thermus thermophilus (strain hb8 / atcc 27634 / dsm 579) (bacteria)
KeywordsRIBOSOME / ribosome ArfA RF2 trans-translation 70S 50S 30S rescue termination cryo-EM / ArfA RF2 trans-translation / 70S / 50S / 30S / rescue termination / cryo-EM / ArfA / RF2 / trans-translation

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