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Structure paper

TitleCharacterization and Crystal Structure of a Robust Cyclohexanone Monooxygenase.
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 55, Page 15852-15855, Year 2016
Publish dateOct 6, 2016 (structure data deposition date)
AuthorsRomero, E. / Castellanos, J.R. / Mattevi, A. / Fraaije, M.W.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:27873437
MethodsX-ray diffraction
Resolution1.22 - 1.6 Å
Structure data

PDB-5m0z:
Cyclohexanone Monooxygenase from T. municipale: reduced enzyme bound to NADP+
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-5m10:
Crystal structure of cyclohexanone monooxygenase from Thermocrispum municipale in the oxidised state with a bound nicotinamide.
Method: X-RAY DIFFRACTION / Resolution: 1.22 Å

Chemicals

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM

ChemComp-NA7:
[(2R,3R,4R,5R)-5-(6-AMINO-9H-PURIN-9-YL)-3-HYDROXY-4-(PHOSPHONOOXY)TETRAHYDROFURAN-2-YL]METHYL [(2R,3S,4S)-3,4-DIHYDROXYTETRAHYDROFURAN-2-YL]METHYL DIHYDROGEN DIPHOSPHATE

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-NCA:
NICOTINAMIDE / medication*YM

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

Source
  • thermocrispum municipale dsm 44069 (bacteria)
  • thermocrispum municipale (bacteria)
KeywordsOXIDOREDUCTASE / Baeyer-Villiger Monooxygenases Flavoenzymes / Cyclohexanone monooxygenase Baeyer-Villiger monooxygenases Flavoenzymes

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