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Structure paper

TitleMembrane protein structure determination by SAD, SIR, or SIRAS phasing in serial femtosecond crystallography using an iododetergent
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 113, Page 13039-13044, Year 2016
Publish dateFeb 10, 2016 (structure data deposition date)
AuthorsNakane, T. / Hanashima, S. / Suzuki, M. / Saiki, H. / Hayashi, T. / Kakinouchi, K. / Sugiyama, S. / Kawatake, S. / Matsuoka, S. / Matsumori, N. ...Nakane, T. / Hanashima, S. / Suzuki, M. / Saiki, H. / Hayashi, T. / Kakinouchi, K. / Sugiyama, S. / Kawatake, S. / Matsuoka, S. / Matsumori, N. / Nango, E. / Kobayashi, J. / Shimamura, T. / Kimura, K. / Mori, C. / Kunishima, N. / Sugahara, M. / Takakyu, Y. / Inoue, S. / Masuda, T. / Hosaka, T. / Tono, K. / Joti, Y. / Kameshima, T. / Hatsui, T. / Yabashi, M. / Inoue, T. / Nureki, O. / Iwata, S. / Murata, M. / Mizohata, E.
External linksProc. Natl. Acad. Sci. USA / PubMed:27799539
MethodsX-ray diffraction
Resolution2.1 - 2.35 Å
Structure data

PDB-5b34:
Serial Femtosecond Crystallography (SFX) of Ground State Bacteriorhodopsin Crystallized from Bicelles in Complex with Iodine-labeled Detergent HAD13a Determined Using 7-keV X-ray Free Electron Laser (XFEL) at SACLA
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-5b35:
Serial Femtosecond Crystallography (SFX) of Ground State Bacteriorhodopsin Crystallized from Bicelles Determined Using 7-keV X-ray Free Electron Laser (XFEL) at SACLA
Method: X-RAY DIFFRACTION / Resolution: 2.35 Å

Chemicals

ChemComp-RET:
RETINAL

ChemComp-4QL:
2,4,6-tris(iodanyl)-5-(octanoylamino)benzene-1,3-dicarboxylic acid

ChemComp-OCT:
N-OCTANE

ChemComp-D12:
DODECANE

ChemComp-D10:
DECANE

ChemComp-R16:
HEXADECANE

ChemComp-HP6:
HEPTANE

ChemComp-DD9:
nonane

ChemComp-HOH:
WATER

ChemComp-4QM:
(3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-10,13-dimethyl-17-[(2R)-pentan-2-yl]-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthrene-3,7,12-triol

Source
  • halobacterium salinarum (Halophile)
KeywordsPROTON TRANSPORT / Membrane protein / Proton pump / Transport Protein / Retinal / SAD

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