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Structure paper

TitleLarge-Scale Movements of IF3 and tRNA during Bacterial Translation Initiation.
Journal, issue, pagesCell, Vol. 167, Issue 1, Page 133-144.e13, Year 2016
Publish dateSep 22, 2016
AuthorsTanweer Hussain / Jose L Llácer / Brian T Wimberly / Jeffrey S Kieft / V Ramakrishnan /
PubMed AbstractIn bacterial translational initiation, three initiation factors (IFs 1-3) enable the selection of initiator tRNA and the start codon in the P site of the 30S ribosomal subunit. Here, we report 11 ...In bacterial translational initiation, three initiation factors (IFs 1-3) enable the selection of initiator tRNA and the start codon in the P site of the 30S ribosomal subunit. Here, we report 11 single-particle cryo-electron microscopy (cryoEM) reconstructions of the complex of bacterial 30S subunit with initiator tRNA, mRNA, and IFs 1-3, representing different steps along the initiation pathway. IF1 provides key anchoring points for IF2 and IF3, thereby enhancing their activities. IF2 positions a domain in an extended conformation appropriate for capturing the formylmethionyl moiety charged on tRNA. IF3 and tRNA undergo large conformational changes to facilitate the accommodation of the formylmethionyl-tRNA (fMet-tRNA(fMet)) into the P site for start codon recognition.
External linksCell / PubMed:27662086 / PubMed Central
MethodsEM (single particle)
Resolution3.55 - 9.7 Å
Structure data

EMDB-4073, PDB-5lmn:
Structure of bacterial 30S-IF1-IF3-mRNA translation pre-initiation complex (state-1A)
Method: EM (single particle) / Resolution: 3.55 Å

EMDB-4074, PDB-5lmo:
Structure of bacterial 30S-IF1-IF3-mRNA translation pre-initiation complex (state-1B)
Method: EM (single particle) / Resolution: 4.3 Å

EMDB-4075, PDB-5lmp:
Structure of bacterial 30S-IF1-IF3-mRNA translation pre-initiation complex (state-1C)
Method: EM (single particle) / Resolution: 5.35 Å

EMDB-4076, PDB-5lmq:
Structure of bacterial 30S-IF1-IF3-mRNA-tRNA translation pre-initiation complex, open form (state-2A)
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-4077, PDB-5lmr:
Structure of bacterial 30S-IF1-IF3-mRNA-tRNA translation pre-initiation complex(state-2B)
Method: EM (single particle) / Resolution: 4.45 Å

EMDB-4078, PDB-5lms:
Structure of bacterial 30S-IF1-IF3-mRNA-tRNA translation pre-initiation complex(state-2C)
Method: EM (single particle) / Resolution: 5.1 Å

EMDB-4079, PDB-5lmt:
Structure of bacterial 30S-IF1-IF3-mRNA-tRNA translation pre-initiation complex(state-3)
Method: EM (single particle) / Resolution: 4.15 Å

EMDB-4080, PDB-5lmu:
Structure of bacterial 30S-IF3-mRNA-tRNA translation pre-initiation complex, closed form (state-4)
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-4081:
Structure of bacterial 30S-IF1-IF2-IF3-mRNA translation pre-initiation complex (state-I)
Method: EM (single particle) / Resolution: 9.7 Å

EMDB-4082:
Structure of bacterial 30S-IF1-IF2-IF3-mRNA-tRNA translation pre-initiation complex(state-II)
Method: EM (single particle) / Resolution: 8.3 Å

EMDB-4083, PDB-5lmv:
Structure of bacterial 30S-IF1-IF2-IF3-mRNA-tRNA translation pre-initiation complex(state-III)
Method: EM (single particle) / Resolution: 4.9 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-A:
ADENOSINE-5'-MONOPHOSPHATE / Adenosine monophosphate

ChemComp-G:
GUANOSINE-5'-MONOPHOSPHATE / Guanosine monophosphate

ChemComp-U:
URIDINE-5'-MONOPHOSPHATE / Uridine monophosphate

ChemComp-FME:
N-FORMYLMETHIONINE / N-Formylmethionine

Source
  • thermus thermophilus hb8 (bacteria)
  • thermus thermophilus (bacteria)
  • thermus thermophilus (strain hb8 / atcc 27634 / dsm 579) (bacteria)
  • escherichia coli (E. coli)
KeywordsRIBOSOME / translation / initiation factors / 30S / IF1 / IF3 / PIC / Thermus thermophilus / tRNAi / IF2

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