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Structure paper

TitleDe Novo Design of an Allosteric Metalloprotein Assembly with Strained Disulfide Bonds.
Journal, issue, pagesJ. Am. Chem. Soc., Vol. 138, Page 13163-13166, Year 2016
Publish dateAug 2, 2016 (structure data deposition date)
AuthorsChurchfield, L.A. / Medina-Morales, A. / Brodin, J.D. / Perez, A. / Tezcan, F.A.
External linksJ. Am. Chem. Soc. / PubMed:27649076
MethodsX-ray diffraction
Resolution2.1 - 2.4 Å
Structure data

PDB-5l31:
Crystal structure of an engineered metal-free RIDC1 variant containing five disulfide bonds.
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-5l32:
Crystal structure of the Zn-RIDC1 complex bearing six interfacial disulfide bonds
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

Chemicals

ChemComp-HEC:
HEME C

ChemComp-NA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-P6G:
HEXAETHYLENE GLYCOL / precipitant*YM

ChemComp-ZN:
Unknown entry

Source
  • escherichia coli (E. coli)
KeywordsELECTRON TRANSPORT / engineered protein / cytochrome / complex / METAL BINDING PROTEIN / protein engineering / metalloprotein / disulfide bonding

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