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Structure paper

TitleMolecular basis of cobalamin-dependent RNA modification.
Journal, issue, pagesNucleic Acids Res., Vol. 44, Page 9965-9976, Year 2016
Publish dateAug 2, 2015 (structure data deposition date)
AuthorsDowling, D.P. / Miles, Z.D. / Kohrer, C. / Maiocco, S.J. / Elliott, S.J. / Bandarian, V. / Drennan, C.L.
External linksNucleic Acids Res. / PubMed:27638883
MethodsX-ray diffraction
Resolution1.747 - 2.653 Å
Structure data

PDB-5d08:
Crystal structure of selenomethionine-labeled epoxyqueuosine reductase
Method: X-RAY DIFFRACTION / Resolution: 1.747 Å

PDB-5d0a:
Crystal structure of epoxyqueuosine reductase with cleaved RNA stem loop
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-5d0b:
Crystal structure of epoxyqueuosine reductase with a tRNA-TYR epoxyqueuosine-modified tRNA stem loop
Method: X-RAY DIFFRACTION / Resolution: 2.645 Å

PDB-5t8y:
Structure of epoxyqueuosine reductase from Bacillus subtilis with the Asp134 catalytic loop swung out of the active site.
Method: X-RAY DIFFRACTION / Resolution: 2.653 Å

Chemicals

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-B12:
COBALAMIN

ChemComp-GOL:
GLYCEROL

ChemComp-PO4:
PHOSPHATE ION

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

Source
  • bacillus subtilis (strain 168) (bacteria)
  • escherichia coli (E. coli)
KeywordsOXIDOREDUCTASE / B12 / tRNA modification / HEAT-domain / queuosine / oxidoreductase/RNA / oxidoreductase-RNA complex / cobalamin / iron sulfur cluster / tRNA modifying enzyme

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