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Structure paper

TitleStructural studies on KijD1, a sugar C-3'-methyltransferase.
Journal, issue, pagesProtein Sci., Vol. 25, Page 2282-2289, Year 2016
Publish dateSep 1, 2016 (structure data deposition date)
AuthorsDow, G.T. / Thoden, J.B. / Holden, H.M.
External linksProtein Sci. / PubMed:27595766
MethodsX-ray diffraction
Resolution1.6 - 2 Å
Structure data

PDB-5t64:
X-ray structure of the C3-methyltransferase KijD1 from Actinomadura kijaniata in complex with TDP and SAH
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-5t67:
x-ray structure of the KijD1 C3-methyltransferase from Actinomadura kijaniata in complex with SAH and dTDP-sugar product
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-5t6b:
X-ray structure of the KijD1 C3-methyltransfeerase, converted to monomeric form
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE

ChemComp-MG:
Unknown entry

ChemComp-TMP:
THYMIDINE-5'-PHOSPHATE

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

ChemComp-JHZ:
(2R,4S,6R)-4-amino-4,6-dimethyl-5-oxotetrahydro-2H-pyran-2-yl [(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl dihydrogen diphosphate (non-preferred name)

ChemComp-CL:
Unknown entry

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

Source
  • actinomadura kijaniata (bacteria)
KeywordsTRANSFERASE / C3-methyltransferase / antitumor / antibiotic / kijanimicin / methyltransferase

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