+検索条件
-Structure paper
タイトル | Asymmetric cryo-EM reconstruction of phage MS2 reveals genome structure in situ. |
---|---|
ジャーナル・号・ページ | Nat Commun, Vol. 7, Page 12524, Year 2016 |
掲載日 | 2016年8月26日 |
著者 | Roman I Koning / Josue Gomez-Blanco / Inara Akopjana / Javier Vargas / Andris Kazaks / Kaspars Tars / José María Carazo / Abraham J Koster / |
PubMed 要旨 | In single-stranded ribonucleic acid (RNA) viruses, virus capsid assembly and genome packaging are intertwined processes. Using cryo-electron microscopy and single particle analysis we determined the ...In single-stranded ribonucleic acid (RNA) viruses, virus capsid assembly and genome packaging are intertwined processes. Using cryo-electron microscopy and single particle analysis we determined the asymmetric virion structure of bacteriophage MS2, which includes 178 copies of the coat protein, a single copy of the A-protein and the RNA genome. This reveals that in situ, the viral RNA genome can adopt a defined conformation. The RNA forms a branched network of stem-loops that almost all allocate near the capsid inner surface, while predominantly binding to coat protein dimers that are located in one-half of the capsid. This suggests that genomic RNA is highly involved in genome packaging and virion assembly. |
リンク | Nat Commun / PubMed:27561669 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 5.0 - 10.5 Å |
構造データ | EMDB-3402: EMDB-3403: EMDB-3404: EMDB-3540: |
由来 |
|