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Structure paper

TitleBiocatalytic Asymmetric Alkene Reduction: Crystal Structure and Characterization of a Double Bond Reductase fromNicotiana tabacum.
Journal, issue, pagesACS Catal, Vol. 3, Page 370-379, Year 2013
Publish dateOct 5, 2012 (structure data deposition date)
AuthorsMansell, D.J. / Toogood, H.S. / Waller, J. / Hughes, J.M. / Levy, C.W. / Gardiner, J.M. / Scrutton, N.S.
External linksACS Catal / PubMed:27547488
MethodsX-ray diffraction
Resolution1.9 - 2.1 Å
Structure data

PDB-4hfj:
X-ray Crystal Structure of a Double Bond Reductase from Nicotiana tabacum
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-4hfm:
X-ray Crystal Structure of a NADP(H)-bound Double Bond Reductase from Nicotiana tabacum
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-4hfn:
X-ray Crystal Structure of a Ternary Complex of Double Bond Reductase from Nicotiana tabacum
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-CIY:
(2E)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enal

Source
  • nicotiana tabacum (common tobacco)
KeywordsOXIDOREDUCTASE / Rossmann fold / twisted b-barrel / Alkene reduction

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