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Structure paper

TitleCellular Cholesterol Directly Activates Smoothened in Hedgehog Signaling.
Journal, issue, pagesCell, Vol. 166, Page 1176-1187.e14, Year 2016
Publish dateJul 25, 2016 (structure data deposition date)
AuthorsHuang, P. / Nedelcu, D. / Watanabe, M. / Jao, C. / Kim, Y. / Liu, J. / Salic, A.
External linksCell / PubMed:27545348
MethodsX-ray diffraction
Resolution1.332 - 2.484 Å
Structure data

PDB-5kzv:
Crystal structure of the xenopus Smoothened cysteine-rich domain (CRD) in complex with 20(S)-hydroxycholesterol
Method: X-RAY DIFFRACTION / Resolution: 1.616 Å

PDB-5kzy:
Crystal structure of the xenopus Smoothened cysteine-rich domain (CRD) in complex with cyclopamine
Method: X-RAY DIFFRACTION / Resolution: 2.484 Å

PDB-5kzz:
Crystal structure of the xenopus Smoothened cysteine-rich domain (CRD) in its apo-form
Method: X-RAY DIFFRACTION / Resolution: 1.332 Å

Chemicals

ChemComp-HCD:
(3alpha,8alpha)-cholest-5-ene-3,20-diol

ChemComp-HOH:
WATER

ChemComp-CY8:
Cyclopamine / alkaloid*YM

ChemComp-ACT:
ACETATE ION

ChemComp-ZN:
Unknown entry

ChemComp-GOL:
GLYCEROL

Source
  • xenopus laevis (African clawed frog)
KeywordsSIGNALING PROTEIN / Hedgehog signaling / GPCR / cysteine-rich domain / sterol

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