EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Uncleaved prefusion-optimized gp140 trimers derived from analysis of HIV-1 envelope metastability.
ジャーナル・号・ページ	Nat Commun, Vol. 7, Page 12040, Year 2016
掲載日	2016年6月28日
著者	Leopold Kong / Linling He / Natalia de Val / Nemil Vora / Charles D Morris / Parisa Azadnia / Devin Sok / Bin Zhou / Dennis R Burton / Andrew B Ward / Ian A Wilson / Jiang Zhu /
PubMed 要旨	The trimeric HIV-1 envelope glycoprotein (Env) is critical for host immune recognition and neutralization. Despite advances in trimer design, the roots of Env trimer metastability remain elusive. ...The trimeric HIV-1 envelope glycoprotein (Env) is critical for host immune recognition and neutralization. Despite advances in trimer design, the roots of Env trimer metastability remain elusive. Here we investigate the contribution of two Env regions to metastability. First, we computationally redesign a largely disordered bend in heptad region 1 (HR1) of SOSIP trimers that connects the long, central HR1 helix to the fusion peptide, substantially improving the yield of soluble, well-folded trimers. Structural and antigenic analyses of two distinct HR1 redesigns confirm that redesigned Env closely mimics the native, prefusion trimer with a more stable gp41. Next, we replace the cleavage site between gp120 and gp41 with various linkers in the context of an HR1 redesign. Electron microscopy reveals a potential fusion intermediate state for uncleaved trimers containing short but not long linkers. Together, these results outline a general approach for stabilization of Env trimers from diverse HIV-1 strains.
リンク	Nat Commun / PubMed:27349805 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	6.308 - 22.0 Å
構造データ	EMDB-6590: CSF trimer in closed conformation 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-6591: CSF trimer in a post-fusion or intermediate state 手法: EM (単粒子) / 解像度: 17.0 Å EMDB-6592: CSF_closed_trimer in complex with the bnAb PGV04 手法: EM (単粒子) / 解像度: 21.0 Å EMDB-6593: CSF_trimer in a post-fusion or intermediate conformation bound to the bnAb PGV04 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-6621: HIV-1 CSF-SOS envelope glycoprotein trimer 手法: EM (単粒子) / 解像度: 21.0 Å EMDB-6622: HIV-1 CSF-SOS envelope glycoprotein trimer bound to bnAb PGV04 手法: EM (単粒子) / 解像度: 21.0 Å EMDB-6623: HIV-1 CSL envelope glycoprotein trimer 手法: EM (単粒子) / 解像度: 17.0 Å EMDB-6624: HIV-1 CSL envelope glycoprotein trimer bound to bnAb PGV04 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-6625: HIV-1 CSL-SOS envelope glycoprotein trimer 手法: EM (単粒子) / 解像度: 21.0 Å EMDB-6626: HIV-1 CSL-SOS envelope glycoprotein trimer bound to bnAb PGV04 手法: EM (単粒子) / 解像度: 22.0 Å EMDB-6627: HIV-1 HR1 redesign 1 envelope glycoprotein trimer 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-6628: HIV-1 HR1 redesign 1 envelope glycoprotein trimer bound to bnAb PGV04 手法: EM (単粒子) / 解像度: 19.0 Å PDB-5js9: Uncleaved prefusion optimized gp140 trimer with an engineered 8-residue HR1 turn bound to broadly neutralizing antibodies 8ANC195 and PGT128 手法: X-RAY DIFFRACTION / 解像度: 6.918 Å PDB-5jsa: Uncleaved prefusion optimized gp140 trimer with an engineered 10-residue HR1 turn bound to broadly neutralizing antibodies 8ANC195 and PGT128 手法: X-RAY DIFFRACTION / 解像度: 6.308 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン
由来	Human immunodeficiency virus (ヒト免疫不全ウイルス) homo sapiens (ヒト) human immunodeficiency virus 1 (ヒト免疫不全ウイルス)
キーワード	VIRAL PROTEIN/IMMUNE SYSTEM / HIV-1 trimer / UFO / SOSIP / vaccine / VIRAL PROTEIN-IMMUNE SYSTEM complex