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TitleBiosynthetic potential of sesquiterpene synthases: product profiles of Egyptian Henbane premnaspirodiene synthase and related mutants.
Journal, issue, pagesJ. Antibiot., Vol. 69, Page 524-533, Year 2016
Publish dateMar 3, 2016 (structure data deposition date)
AuthorsKoo, H.J. / Vickery, C.R. / Xu, Y. / Louie, G.V. / O'Maille, P.E. / Bowman, M. / Nartey, C.M. / Burkart, M.D. / Noel, J.P.
External linksJ. Antibiot. / PubMed:27328867
MethodsX-ray diffraction
Resolution2.1 - 2.45 Å
Structure data

PDB-5ik0:
Tobacco 5-epi-aristolochene synthase with FPP
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-5ik6:
Tobacco 5-epi-aristolochene synthase with germacrene A and PPi
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-5ik9:
Tobacco 5-epi-aristolochene with farnesyl monophosphate
Method: X-RAY DIFFRACTION / Resolution: 2.23 Å

PDB-5ika:
Tobacco 5-epi-aristolochene synthase with PPi
Method: X-RAY DIFFRACTION / Resolution: 2.45 Å

PDB-5ikh:
Tobacco 5-epi-aristolochene synthase M4 mutant with (-)-premnaspirodiene
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-FPP:
FARNESYL DIPHOSPHATE

ChemComp-HOH:
WATER

ChemComp-CRE:
GERMACRENE A / Antimicrobial*YM

ChemComp-DPO:
DIPHOSPHATE

ChemComp-FJP:
(2E,6Z)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl dihydrogen phosphate

ChemComp-6BW:
(2R,5S,10R)-6,10-dimethyl-2-(prop-1-en-2-yl)spiro[4.5]dec-6-ene

Source
  • nicotiana tabacum (common tobacco)
KeywordsLYASE / terpene synthase / TEAS / FPP / germacrene A / diphosphate / FMP / TEAS-M4 / premnaspirodiene

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