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TitleCrystal structure of norcoclaurine-6-O-methyltransferase, a key rate-limiting step in the synthesis of benzylisoquinoline alkaloids.
Journal, issue, pagesPlant J., Vol. 87, Page 641-653, Year 2016
Publish dateFeb 23, 2016 (structure data deposition date)
AuthorsRobin, A.Y. / Giustini, C. / Graindorge, M. / Matringe, M. / Dumas, R.
External linksPlant J. / PubMed:27232113
MethodsX-ray diffraction
Resolution1.6 - 2.6 Å
Structure data

PDB-5icc:
Crystal structure of (S)-norcoclaurine 6-O-methyltransferase with S-adenosyl-L-homocysteine
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-5ice:
Crystal structure of (S)-norcoclaurine 6-O-methyltransferase with S-adenosyl-L-homocysteine and norlaudanosoline
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-5icf:
Crystal structure of (S)-norcoclaurine 6-O-methyltransferase with S-adenosyl-L-homocysteine and sanguinarine
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-5icg:
Crystal structure of apo (S)-norcoclaurine 6-O-methyltransferase
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

Chemicals

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE

ChemComp-K:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

ChemComp-2H4:
(1S)-1-(3,4-dihydroxybenzyl)-1,2,3,4-tetrahydroisoquinoline-6,7-diol

ChemComp-SAU:
13-methyl[1,3]benzodioxolo[5,6-c][1,3]dioxolo[4,5-i]phenanthridin-13-ium / alkaloid*YM

ChemComp-DMS:
DIMETHYL SULFOXIDE / DMSO, precipitant*YM

Source
  • thalictrum flavum subsp. glaucum (plant)
KeywordsTRANSFERASE / methyltransferase / benzylisoquinoline alkaloid

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