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Structure paper

TitleInsights into RNA binding by the anticancer drug cisplatin from the crystal structure of cisplatin-modified ribosome.
Journal, issue, pagesNucleic Acids Res., Vol. 44, Page 4978-4987, Year 2016
Publish dateMar 31, 2016 (structure data deposition date)
AuthorsMelnikov, S.V. / Soll, D. / Steitz, T.A. / Polikanov, Y.S.
External linksNucleic Acids Res. / PubMed:27079977
MethodsX-ray diffraction
Resolution2.6 - 2.8 Å
Structure data

PDB-5j4b:
Crystal structure of the Thermus thermophilus 70S ribosome in complex with cisplatin (co-crystallized) and bound to mRNA and A-, P- and E-site tRNAs at 2.6A resolution
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-5j4c:
Crystal structure of the Thermus thermophilus 70S ribosome in complex with cisplatin (soaked) and bound to mRNA and A-, P- and E-site tRNAs at 2.8A resolution
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-K:
Unknown entry

ChemComp-CPT:
Cisplatin / medication, chemotherapy*YM

ChemComp-ZN:
Unknown entry

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-HOH:
WATER

Source
  • escherichia coli (E. coli)
  • thermus thermophilus hb8 (bacteria)
  • thermus thermophilus (strain hb8 / atcc 27634 / dsm 579) (bacteria)
  • synthetic construct (others)
KeywordsRIBOSOME / cisplatin / anticancer drug / cytotoxicity / inhibition of translation / translocation / mRNA channel / GTPase activating center

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