EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Three-dimensional structural dynamics and fluctuations of DNA-nanogold conjugates by individual-particle electron tomography.
ジャーナル・号・ページ	Nat Commun, Vol. 7, Page 11083, Year 2016
掲載日	2016年3月30日
著者	Lei Zhang / Dongsheng Lei / Jessica M Smith / Meng Zhang / Huimin Tong / Xing Zhang / Zhuoyang Lu / Jiankang Liu / A Paul Alivisatos / Gang Ren /
PubMed 要旨	DNA base pairing has been used for many years to direct the arrangement of inorganic nanocrystals into small groupings and arrays with tailored optical and electrical properties. The control of DNA- ...DNA base pairing has been used for many years to direct the arrangement of inorganic nanocrystals into small groupings and arrays with tailored optical and electrical properties. The control of DNA-mediated assembly depends crucially on a better understanding of three-dimensional structure of DNA-nanocrystal-hybridized building blocks. Existing techniques do not allow for structural determination of these flexible and heterogeneous samples. Here we report cryo-electron microscopy and negative-staining electron tomography approaches to image, and three-dimensionally reconstruct a single DNA-nanogold conjugate, an 84-bp double-stranded DNA with two 5-nm nanogold particles for potential substrates in plasmon-coupling experiments. By individual-particle electron tomography reconstruction, we obtain 14 density maps at ∼2-nm resolution. Using these maps as constraints, we derive 14 conformations of dsDNA by molecular dynamics simulations. The conformational variation is consistent with that from liquid solution, suggesting that individual-particle electron tomography could be an expected approach to study DNA-assembling and flexible protein structure and dynamics.
リンク	Nat Commun / PubMed:27025159 / PubMed Central
手法	EM (トモグラフィー)
解像度	14.7 - 23.3 Å
構造データ	EMDB-2948: Three Dimensional Dynamics and Fluctuations of DNA-Nanogold Dimers by Individual-Particle Electron Tomography 手法: EM (トモグラフィー) / 解像度: 14.7 Å EMDB-2949: Three Dimensional Dynamics and Fluctuations of DNA-Nanogold Dimers by Individual-Particle Electron Tomography 手法: EM (トモグラフィー) / 解像度: 17.1 Å EMDB-2950: Three Dimensional Dynamics and Fluctuations of DNA-Nanogold Dimers by Individual-Particle Electron Tomography 手法: EM (トモグラフィー) / 解像度: 17.7 Å EMDB-2951: Three Dimensional Dynamics and Fluctuations of DNA-Nanogold Dimers by Individual-Particle Electron Tomography 手法: EM (トモグラフィー) / 解像度: 15.7 Å EMDB-2952: Three Dimensional Dynamics and Fluctuations of DNA-Nanogold Dimers by Individual-Particle Electron Tomography 手法: EM (トモグラフィー) / 解像度: 18.9 Å EMDB-2953: Three Dimensional Dynamics and Fluctuations of DNA-Nanogold Dimers by Individual-Particle Electron Tomography 手法: EM (トモグラフィー) / 解像度: 15.2 Å EMDB-2954: Three Dimensional Dynamics and Fluctuations of DNA-Nanogold Dimers by Individual-Particle Electron Tomography 手法: EM (トモグラフィー) / 解像度: 17.7 Å EMDB-2955: Three Dimensional Dynamics and Fluctuations of DNA-Nanogold Dimers by Individual-Particle Electron Tomography 手法: EM (トモグラフィー) / 解像度: 23.3 Å EMDB-2956: Three Dimensional Dynamics and Fluctuations of DNA-Nanogold Dimers by Individual-Particle Electron Tomography 手法: EM (トモグラフィー) / 解像度: 17.3 Å EMDB-2957: Three Dimensional Dynamics and Fluctuations of DNA-Nanogold Dimers by Individual-Particle Electron Tomography 手法: EM (トモグラフィー) / 解像度: 17.8 Å EMDB-2958: Three Dimensional Dynamics and Fluctuations of DNA-Nanogold Dimers by Individual-Particle Electron Tomography 手法: EM (トモグラフィー) / 解像度: 17.7 Å EMDB-2959: Three Dimensional Dynamics and Fluctuations of DNA-Nanogold Dimers by Individual-Particle Electron Tomography 手法: EM (トモグラフィー) / 解像度: 17.8 Å EMDB-2960: Three Dimensional Dynamics and Fluctuations of DNA-Nanogold Dimers by Individual-Particle Electron Tomography 手法: EM (トモグラフィー) / 解像度: 17.9 Å EMDB-2961: Three Dimensional Dynamics and Fluctuations of DNA-Nanogold Dimers by Individual-Particle Electron Tomography 手法: EM (トモグラフィー) / 解像度: 14.7 Å
由来	synthetic construct (人工物)