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-Structure paper
タイトル | Cryo-EM single particle analysis with the Volta phase plate. |
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ジャーナル・号・ページ | Elife, Vol. 5, Year 2016 |
掲載日 | 2016年3月7日 |
![]() | Radostin Danev / Wolfgang Baumeister / ![]() |
PubMed 要旨 | We present a method for in-focus data acquisition with a phase plate that enables near-atomic resolution single particle reconstructions. Accurate focusing is the determining factor for obtaining ...We present a method for in-focus data acquisition with a phase plate that enables near-atomic resolution single particle reconstructions. Accurate focusing is the determining factor for obtaining high quality data. A double-area focusing strategy was implemented in order to achieve the required precision. With this approach we obtained a 3.2 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome. The phase plate matches or slightly exceeds the performance of the conventional defocus approach. Spherical aberration becomes a limiting factor for achieving resolutions below 3 Å with in-focus phase plate images. The phase plate could enable single particle analysis of challenging samples in terms of small size, heterogeneity and flexibility that are difficult to solve by the conventional defocus approach. |
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手法 | EM (単粒子) |
解像度 | 3.1 - 3.2 Å |
構造データ | ![]() EMDB-3347: ![]() EMDB-3348: |
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