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Structure paper

TitleMultiplexed protein-DNA cross-linking: Scrunching in transcription start site selection.
Journal, issue, pagesScience, Vol. 351, Page 1090-1093, Year 2016
Publish dateSep 29, 2015 (structure data deposition date)
AuthorsWinkelman, J.T. / Vvedenskaya, I.O. / Zhang, Y. / Bird, J.G. / Taylor, D.M. / Gourse, R.L. / Ebright, R.H. / Nickels, B.E.
External linksScience / PubMed:26941320
MethodsX-ray diffraction
Resolution3.2 - 3.3 Å
Structure data

PDB-5e17:
T. thermophilus transcription initiation complex having a RRR discriminator sequence and a nontemplate-strand length corresponding to TSS selection at position 7 (RPo-GGG-7)
Method: X-RAY DIFFRACTION / Resolution: 3.2 Å

PDB-5e18:
T. thermophilus transcription initiation complex having a YYY discriminator sequence and a nontemplate-strand length corresponding to TSS selection at position 8 (RPo-CCC-8)
Method: X-RAY DIFFRACTION / Resolution: 3.3 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER

Source
  • thermus thermophilus (strain hb8 / atcc 27634 / dsm 579) (bacteria)
  • thermus thermophilus (bacteria)
Keywordstranscription/dna/rna / DNA / Single-Stranded / DNA-Directed RNA Polymerases / Gene Expression Regulation / Bacterial / Promoter Regions / Protein Conformation / Sigma Factor / Thermus thermophilus / Transcription Initiation / start site selection / promoter escape / initial transcript / abortive product / sigma finger / transcription-dna-rna complex / discriminator / consensus / RNA / abortive / sigma / transcription start site / scrunching

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