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TitleDocking-complex-independent alignment of Chlamydomonas outer dynein arms with 24-nm periodicity in vitro.
Journal, issue, pagesJ Cell Sci, Vol. 129, Issue 8, Page 1547-1551, Year 2016
Publish dateApr 15, 2016
AuthorsToshiyuki Oda / Tatsuki Abe / Haruaki Yanagisawa / Masahide Kikkawa /
PubMed AbstractThe docking complex is a molecular complex necessary for assembly of outer dynein arms (ODAs) on the axonemal doublet microtubules (DMTs) in cilia and flagella. The docking complex is hypothesized to ...The docking complex is a molecular complex necessary for assembly of outer dynein arms (ODAs) on the axonemal doublet microtubules (DMTs) in cilia and flagella. The docking complex is hypothesized to be a 24-nm molecular ruler because ODAs align along the DMTs with 24-nm periodicity. In this study, we rigorously tested this hypothesis using structural and genetic methods. We found that the ODAs can bind to DMTs and porcine microtubules with 24-nm periodicities even in the absence of the docking complexin vitro Using cryo-electron tomography and structural labeling, we observed that the docking complex took an unexpectedly flexible conformation and did not lie along the length of DMTs. In the absence of docking complex, ODAs were released from the DMT at relatively low ionic strength conditions, suggesting that the docking complex strengthens the electrostatic interactions between the ODA and DMT. Based on these results, we conclude that the docking complex serves as a flexible stabilizer of the ODA rather than as a molecular ruler.
External linksJ Cell Sci / PubMed:26933181
MethodsEM (subtomogram averaging)
Resolution45.0 - 49.0 Å
Structure data

EMDB-6504:
Docking complex-independent alignment of outer dynein arms with 24-nm periodicity
Method: EM (subtomogram averaging) / Resolution: 47.0 Å

EMDB-6505:
Docking complex-independent alignment of outer dynein arms with 24-nm periodicity
Method: EM (subtomogram averaging) / Resolution: 48.0 Å

EMDB-6506:
Docking complex-independent alignment of outer dynein arms with 24-nm periodicity
Method: EM (subtomogram averaging) / Resolution: 45.0 Å

EMDB-6507:
Docking complex-independent alignment of outer dynein arms with 24-nm periodicity
Method: EM (subtomogram averaging) / Resolution: 49.0 Å

EMDB-6508:
Docking complex-independent alignment of outer dynein arms with 24-nm periodicity
Method: EM (subtomogram averaging) / Resolution: 47.0 Å

EMDB-6509:
Docking complex-independent alignment of outer dynein arms with 24-nm periodicity
Method: EM (subtomogram averaging) / Resolution: 46.0 Å

EMDB-6510:
Docking complex-independent alignment of outer dynein arms with 24-nm periodicity
Method: EM (subtomogram averaging) / Resolution: 47.0 Å

EMDB-6511:
Docking complex-independent alignment of outer dynein arms with 24-nm periodicity
Method: EM (subtomogram averaging) / Resolution: 49.0 Å

Source
  • Chlamydomonas reinhardtii (plant)

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