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Structure paper

TitleAntigen clasping by two antigen-binding sites of an exceptionally specific antibody for histone methylation.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 113, Page 2092-2097, Year 2016
Publish dateFeb 27, 2015 (structure data deposition date)
AuthorsHattori, T. / Lai, D. / Dementieva, I.S. / Montano, S.P. / Kurosawa, K. / Zheng, Y. / Akin, L.R. / Swist-Rosowska, K.M. / Grzybowski, A.T. / Koide, A. ...Hattori, T. / Lai, D. / Dementieva, I.S. / Montano, S.P. / Kurosawa, K. / Zheng, Y. / Akin, L.R. / Swist-Rosowska, K.M. / Grzybowski, A.T. / Koide, A. / Krajewski, K. / Strahl, B.D. / Kelleher, N.L. / Ruthenburg, A.J. / Koide, S.
External linksProc. Natl. Acad. Sci. USA / PubMed:26862167
MethodsX-ray diffraction
Resolution1.9 - 2.53 Å
Structure data

PDB-4yhp:
Crystal structure of 309M3-B Fab in complex with H3K9me3 peptide
Method: X-RAY DIFFRACTION / Resolution: 2.53 Å

PDB-4yhy:
Crystal structure of 309M3-B in complex with trimethylated Lys
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-4yhz:
Crystal structure of 304M3-B Fab in complex with H3K4me3 peptide
Method: X-RAY DIFFRACTION / Resolution: 2.304 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-M3L:
N-TRIMETHYLLYSINE

ChemComp-GOL:
GLYCEROL

ChemComp-SO4:
SULFATE ION

Source
  • homo sapiens (human)
  • synthetic construct (others)
KeywordsIMMUNE SYSTEM / Antibody / Fab / Head-to-head dimerization / H3K9me3 / Trimethylated Lys / H3K4me3

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