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Structure paper

TitleRational steering of insulin binding specificity by intra-chain chemical crosslinking.
Journal, issue, pagesSci Rep, Vol. 6, Page 19431-19431, Year 2016
Publish dateMay 15, 2015 (structure data deposition date)
AuthorsVikova, J. / Collinsova, M. / Kletvikova, E. / Budesinsky, M. / Kaplan, V. / Zakova, L. / Veverka, V. / Hexnerova, R. / Avino, R.J. / Strakova, J. ...Vikova, J. / Collinsova, M. / Kletvikova, E. / Budesinsky, M. / Kaplan, V. / Zakova, L. / Veverka, V. / Hexnerova, R. / Avino, R.J. / Strakova, J. / Selicharova, I. / Vanek, V. / Wright, D.W. / Watson, C.J. / Turkenburg, J.P. / Brzozowski, A.M. / Jiracek, J.
External linksSci Rep / PubMed:26792393
MethodsNMR (solution) / X-ray diffraction
Resolution1.54 - 1.9 Å
Structure data

PDB-2n2v:
Solution structure of [B26-B29 triazole cross-linked]-insulin analogue at pH 1.9
Method: SOLUTION NMR

PDB-2n2w:
Solution structure of [B26-B29 triazole cross-linked]-insulin analogue at pH 8.0
Method: SOLUTION NMR

PDB-2n2x:
Solution structure of [GlyB24,B27-B29 triazole cross-linked]-insulin analogue at pH 1.9
Method: SOLUTION NMR

PDB-5boq:
Human insulin with intra-chain chemical crosslink between modified B24 and B29
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-5bpo:
Human insulin with intra-chain chemical crosslink between modified B27 and B29
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-5bqq:
Human insulin with intra-chain chemical crosslink between modified B27 and B30
Method: X-RAY DIFFRACTION / Resolution: 1.54 Å

Chemicals

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-HOH:
WATER / Water

ChemComp-IPH:
PHENOL / Phenol

ChemComp-ZN:
Unknown entry

ChemComp-CL:
Unknown entry / Chloride

Source
  • homo sapiens (human)
KeywordsHORMONE / Chemical crosslink / B24-B29 / specificity

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