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TitleThe Combination of X-Ray Crystallography and Cryo-Electron Microscopy Provides Insight into the Overall Architecture of the Dodecameric Rvb1/Rvb2 Complex.
Journal, issue, pagesPLoS One, Vol. 11, Issue 1, Page e0146457, Year 2016
Publish dateJan 8, 2016
AuthorsNoella Silva-Martin / María I Daudén / Sebastian Glatt / Niklas A Hoffmann / Panagiotis Kastritis / Peer Bork / Martin Beck / Christoph W Müller /
PubMed AbstractThe Rvb1/Rvb2 complex is an essential component of many cellular pathways. The Rvb1/Rvb2 complex forms a dodecameric assembly where six copies of each subunit form two heterohexameric rings. However, ...The Rvb1/Rvb2 complex is an essential component of many cellular pathways. The Rvb1/Rvb2 complex forms a dodecameric assembly where six copies of each subunit form two heterohexameric rings. However, due to conformational variability, the way the two rings pack together is still not fully understood. Here, we present the crystal structure and two cryo-electron microscopy reconstructions of the dodecameric, full-length Rvb1/Rvb2 complex, all showing that the interaction between the two heterohexameric rings is mediated through the Rvb1/Rvb2-specific domain II. Two conformations of the Rvb1/Rvb2 dodecamer are present in solution: a stretched conformation also present in the crystal, and a compact conformation. Novel asymmetric features observed in the reconstruction of the compact conformation provide additional insight into the plasticity of the Rvb1/Rvb2 complex.
External linksPLoS One / PubMed:26745716 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution2.901 - 21.4 Å
Structure data

EMDB-3242:
Dodecameric Chaetomium thermophilum Rvb1/Rvb2 complex in compact conformation
Method: EM (single particle) / Resolution: 12.3 Å

EMDB-3243:
Dodecameric Chaetomium thermophilum Rvb1/Rvb2 complex in stretched conformation
Method: EM (single particle) / Resolution: 21.4 Å

PDB-5fm6:
Double-heterohexameric rings of full-length Rvb1(ADP)Rvb2(apo)
Method: X-RAY DIFFRACTION / Resolution: 2.997 Å

PDB-5fm7:
Double-heterohexameric rings of full-length Rvb1(ADP)Rvb2(ADP)
Method: X-RAY DIFFRACTION / Resolution: 2.901 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-PO4:
PHOSPHATE ION

Source
  • chaetomium thermophilum (fungus)
KeywordsATP BINDING PROTEIN / UNKNOWN FUNCTION / RVB1 / RVB2 / ADP

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