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TitleSubtle structural changes in the Asp251Gly/Gln307His P450 BM3 mutant responsible for new activity toward diclofenac, tolbutamide and ibuprofen.
Journal, issue, pagesArch. Biochem. Biophys., Vol. 602, Page 106-115, Year 2016
Publish dateSep 25, 2015 (structure data deposition date)
AuthorsDi Nardo, G. / Dell'Angelo, V. / Catucci, G. / Sadeghi, S.J. / Gilardi, G.
External linksArch. Biochem. Biophys. / PubMed:26718083
MethodsX-ray diffraction
Resolution1.967 - 2.4 Å
Structure data

PDB-5dyp:
Crystal structure of Asp251Gly/Gln307His mutant of cytochrome P450 BM3
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-5dyz:
Crystal structure of Asp251Gly/Gln307His mutant of cytochrome P450 BM3 in complex with N-palmitoylglycine
Method: X-RAY DIFFRACTION / Resolution: 1.967 Å

Chemicals

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-HOH:
WATER

ChemComp-140:
N-PALMITOYLGLYCINE

Source
  • bacillus megaterium (bacteria)
KeywordsOXIDOREDUCTASE / cytochrome P450 / random mutagenesis / drug metabolism / substrate

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