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TitleStructure of the hypusinylated eukaryotic translation factor eIF-5A bound to the ribosome.
Journal, issue, pagesNucleic Acids Res, Vol. 44, Issue 4, Page 1944-1951, Year 2016
Publish dateFeb 29, 2016
AuthorsChristian Schmidt / Thomas Becker / André Heuer / Katharina Braunger / Vivekanandan Shanmuganathan / Markus Pech / Otto Berninghausen / Daniel N Wilson / Roland Beckmann /
PubMed AbstractDuring protein synthesis, ribosomes become stalled on polyproline-containing sequences, unless they are rescued in archaea and eukaryotes by the initiation factor 5A (a/eIF-5A) and in bacteria by the ...During protein synthesis, ribosomes become stalled on polyproline-containing sequences, unless they are rescued in archaea and eukaryotes by the initiation factor 5A (a/eIF-5A) and in bacteria by the homologous protein EF-P. While a structure of EF-P bound to the 70S ribosome exists, structural insight into eIF-5A on the 80S ribosome has been lacking. Here we present a cryo-electron microscopy reconstruction of eIF-5A bound to the yeast 80S ribosome at 3.9 Å resolution. The structure reveals that the unique and functionally essential post-translational hypusine modification reaches toward the peptidyltransferase center of the ribosome, where the hypusine moiety contacts A76 of the CCA-end of the P-site tRNA. These findings would support a model whereby eIF-5A stimulates peptide bond formation on polyproline-stalled ribosomes by stabilizing and orienting the CCA-end of the P-tRNA, rather than by directly contributing to the catalysis.
External linksNucleic Acids Res / PubMed:26715760 / PubMed Central
MethodsEM (single particle)
Resolution3.88 Å
Structure data

3227

5gak

EMDB-3227: Cryo-EM map of a native 80S-ribosome-eIF-5A complex
PDB-5gak: Yeast 60S ribosomal subunit with A-site tRNA, P-site tRNA and eIF-5A
Method: EM (single particle) / Resolution: 3.88 Å

Chemicals

ChemComp-3HE:
4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione / Cycloheximide

Source
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsRIBOSOME / translation / hypusine

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