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TitleStructure of a bacterial type III secretion system in contact with a host membrane in situ.
Journal, issue, pagesNat Commun, Vol. 6, Page 10114, Year 2015
Publish dateDec 11, 2015
AuthorsAndrea Nans / Mikhail Kudryashev / Helen R Saibil / Richard D Hayward /
PubMed AbstractMany bacterial pathogens of animals and plants use a conserved type III secretion system (T3SS) to inject virulence effector proteins directly into eukaryotic cells to subvert host functions. Contact ...Many bacterial pathogens of animals and plants use a conserved type III secretion system (T3SS) to inject virulence effector proteins directly into eukaryotic cells to subvert host functions. Contact with host membranes is critical for T3SS activation, yet little is known about T3SS architecture in this state or the conformational changes that drive effector translocation. Here we use cryo-electron tomography and sub-tomogram averaging to derive the intact structure of the primordial Chlamydia trachomatis T3SS in the presence and absence of host membrane contact. Comparison of the averaged structures demonstrates a marked compaction of the basal body (4 nm) occurs when the needle tip contacts the host cell membrane. This compaction is coupled to a stabilization of the cytosolic sorting platform-ATPase. Our findings reveal the first structure of a bacterial T3SS from a major human pathogen engaged with a eukaryotic host, and reveal striking 'pump-action' conformational changes that underpin effector injection.
External linksNat Commun / PubMed:26656452 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution33.0 - 38.0 Å
Structure data

EMDB-3216:
In situ sub-tomogram average of the host-free Chlamydia trachomatis type III secretion system
Method: EM (subtomogram averaging) / Resolution: 33.0 Å

EMDB-3217:
In situ sub-tomogram average of the host-contact Chlamydia trachomatis type III secretion system
Method: EM (subtomogram averaging) / Resolution: 38.0 Å

Source
  • Chlamydia trachomatis (bacteria)

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