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Structure paper

TitleStructural determinants of reductive terpene cyclization in iridoid biosynthesis.
Journal, issue, pagesNat. Chem. Biol., Vol. 12, Page 6-8, Year 2016
Publish dateAug 24, 2015 (structure data deposition date)
AuthorsKries, H. / Caputi, L. / Stevenson, C.E. / Kamileen, M.O. / Sherden, N.H. / Geu-Flores, F. / Lawson, D.M. / O'Connor, S.E.
External linksNat. Chem. Biol. / PubMed:26551396
MethodsX-ray diffraction
Resolution1.4 - 1.9 Å
Structure data

PDB-5dcu:
Iridoid synthase from Catharanthus roseus - ternary complex with NADP+ and triethylene glycol carboxylic acid
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-5dcw:
Iridoid synthase from Catharanthus roseus - ligand free structure
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-5dcy:
Iridoid synthase G150A mutant from Catharanthus roseus - binary complex with NADP+
Method: X-RAY DIFFRACTION / Resolution: 1.45 Å

PDB-5df1:
Iridoid synthase from Catharanthus roseus - ternary complex with NADP+ and geranic acid
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

Chemicals

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-TEG:
[2-(2-hydroxyethoxy)ethoxy]acetic acid

ChemComp-HOH:
WATER

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-58X:
(2E)-3,7-dimethylocta-2,6-dienoic acid

ChemComp-IMD:
IMIDAZOLE

Source
  • catharanthus roseus (Madagascar periwinkle)
KeywordsOXIDOREDUCTASE / Iridoid synthase / short chain dehydrogenase / NADPH-dependent / Catharanthus roseus

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