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TitleA Combination of Structural and Empirical Analyses Delineates the Key Contacts Mediating Stability and Affinity Increases in an Optimized Biotherapeutic Single-chain Fv (scFv).
Journal, issue, pagesJ. Biol. Chem., Vol. 291, Page 1267-1276, Year 2016
Publish dateJun 30, 2015 (structure data deposition date)
AuthorsTu, C. / Terraube, V. / Tam, A.S. / Stochaj, W. / Fennell, B.J. / Lin, L. / Stahl, M. / LaVallie, E.R. / Somers, W. / Finlay, W.J. ...Tu, C. / Terraube, V. / Tam, A.S. / Stochaj, W. / Fennell, B.J. / Lin, L. / Stahl, M. / LaVallie, E.R. / Somers, W. / Finlay, W.J. / Mosyak, L. / Bard, J. / Cunningham, O.
External linksJ. Biol. Chem. / PubMed:26515064
MethodsX-ray diffraction
Resolution2.4 - 2.5 Å
Structure data

PDB-5cba:
3B4 in complex with CXCL13 - 3B4-CXCL13
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-5cbe:
E10 in complex with CXCL13
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

Chemicals

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
KeywordsIMMUNE SYSTEM / anti-CXCL13 / CXCL13 / scFv / E10

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