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Structure paper

TitleCarD uses a minor groove wedge mechanism to stabilize the RNA polymerase open promoter complex.
Journal, issue, pagesElife, Vol. 4, Year 2015
Publish dateDec 15, 2014 (structure data deposition date)
AuthorsBae, B. / Chen, J. / Davis, E. / Leon, K. / Darst, S.A. / Campbell, E.A.
External linksElife / PubMed:26349034
MethodsX-ray diffraction
Resolution2.404 - 4.3 Å
Structure data

PDB-4xax:
Crystal structure of Thermus thermophilus CarD in complex with the Thermus aquaticus RNA polymerase beta1 domain
Method: X-RAY DIFFRACTION / Resolution: 2.404 Å

PDB-4xlr:
Crystal structure of T.aquaticus transcription initiation complex with CarD containing bubble promoter and RNA
Method: X-RAY DIFFRACTION / Resolution: 4.3 Å

PDB-4xls:
Crystal structure of T. aquaticus transcription initiation complex with CarD containing upstream fork promoter.
Method: X-RAY DIFFRACTION / Resolution: 4.01 Å

Chemicals

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • thermus aquaticus (bacteria)
  • thermus thermophilus (strain hb8 / atcc 27634 / dsm 579) (bacteria)
  • thermus thermophilus jl-18 (bacteria)
  • synthetic construct (others)
KeywordsTranscription Regulator / transcription/DNA / protein-DNA complex / Bacterial transcription initiation complex / transcription-DNA complex / TRANSCRIPTION

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