+Search query
-Structure paper
Title | Structural basis for specific recognition of single-stranded RNA by Toll-like receptor 13. |
---|---|
Journal, issue, pages | Nat Struct Mol Biol, Vol. 22, Issue 10, Page 782-787, Year 2015 |
Publish date | Aug 31, 2015 |
Authors | Wen Song / Jia Wang / Zhifu Han / Yifan Zhang / Heqiao Zhang / Weiguang Wang / Junbiao Chang / Bingshu Xia / Shilong Fan / Dekai Zhang / Jiawei Wang / Hong-Wei Wang / Jijie Chai / |
PubMed Abstract | Toll-like receptors (TLRs) have crucial roles in innate immunity, functioning as pattern-recognition receptors. TLR13 recognizes a conserved sequence from bacterial 23S rRNA and then triggers an ...Toll-like receptors (TLRs) have crucial roles in innate immunity, functioning as pattern-recognition receptors. TLR13 recognizes a conserved sequence from bacterial 23S rRNA and then triggers an immune response. Here we report the crystal structure of the mouse TLR13 ectodomain bound by a 13-nt single-stranded (ss) RNA derived from 23S rRNA. The ssRNA induces TLR13 dimerization but assumes a stem-loop-like structure that is completely different from that in the bacterial ribosome but nevertheless is crucial for TLR13 recognition. Most of the RNA nucleotides are splayed out to make base-specific contacts with the concave surface of TLR13, and RNA-specific interactions are important to allow TLR13 to distinguish RNA from DNA. Interestingly, a viral-derived 16-nt ssRNA predicted to form a similar stem-loop-like structure also induces TLR13 activation. Together, our results reveal the structural mechanism of TLR13's sequence- and conformation-specific recognition of ssRNA. |
External links | Nat Struct Mol Biol / PubMed:26323037 |
Methods | EM (single particle) / X-ray diffraction |
Resolution | 2.3 - 4.87 Å |
Structure data | EMDB-3125: PDB-4z0c: |
Chemicals | ChemComp-NAG: ChemComp-HOH: |
Source |
|
Keywords | IMMUNE SYSTEM / immune receptor / Toll-like receptor / ssRNA |