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Structure paper

TitleCatalysis of GTP Hydrolysis by Small GTPases at Atomic Detail by Integration of X-ray Crystallography, Experimental, and Theoretical IR Spectroscopy.
Journal, issue, pagesJ. Biol. Chem., Vol. 290, Page 24079-24090, Year 2015
Publish dateJul 13, 2015 (structure data deposition date)
AuthorsRudack, T. / Jenrich, S. / Brucker, S. / Vetter, I.R. / Gerwert, K. / Kotting, C.
External linksJ. Biol. Chem. / PubMed:26272610
MethodsX-ray diffraction
Resolution1.65 - 3.2 Å
Structure data

PDB-5ciq:
Ran GDP wild type tetragonal crystal form
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

PDB-5cit:
Ran GDP wild type monoclinic crystal form
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-5ciw:
Ran GDP Y39A mutant monoclinic crystal form
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-5cj2:
Ran GDP Y39A mutant triclinic crystal form
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-5cll:
Truncated Ran wild type in complex with GDP-BeF and RanBD1
Method: X-RAY DIFFRACTION / Resolution: 2.45 Å

PDB-5clq:
Ran Y39A in complex with GPPNHP and RanBD1
Method: X-RAY DIFFRACTION / Resolution: 3.2 Å

Chemicals

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-PO4:
PHOSPHATE ION

ChemComp-BEF:
BERYLLIUM TRIFLUORIDE ION

ChemComp-GNP:
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GppNHp, GMPPNP, energy-carrying molecule analogue*YM

ChemComp-SO4:
SULFATE ION

Source
  • homo sapiens (human)
KeywordsHYDROLASE / GTPase / nuclear transport / transport protein / TRANSCRIPTION / GTPase nuclear transport transport protein / Ran binding protein

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