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TitleSpeeding up the product release: a second-sphere contribution from Tyr191 to the reactivity of l-lactate oxidase revealed in crystallographic and kinetic studies of site-directed variants.
Journal, issue, pagesFebs J., Vol. 282, Page 4130-4140, Year 2015
Publish dateMar 4, 2015 (structure data deposition date)
AuthorsStoisser, T. / Klimacek, M. / Wilson, D.K. / Nidetzky, B.
External linksFebs J. / PubMed:26260739
MethodsX-ray diffraction
Resolution1.9 Å
Structure data

PDB-4yl2:
Aerococcus viridans L-lactate oxidase Y191F mutant
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

Chemicals

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

ChemComp-PYR:
PYRUVIC ACID

ChemComp-HOH:
WATER

Source
  • aerococcus viridans (bacteria)
KeywordsOXIDOREDUCTASE / flavin / oxidase / TIM barrel

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