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Structure paper

TitleStructural basis for methyl-donor-dependent and sequence-specific binding to tRNA substrates by knotted methyltransferase TrmD.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 112, Page E4197-E4205, Year 2015
Publish dateMar 20, 2015 (structure data deposition date)
AuthorsIto, T. / Masuda, I. / Yoshida, K. / Goto-Ito, S. / Sekine, S. / Suh, S.W. / Hou, Y.M. / Yokoyama, S.
External linksProc. Natl. Acad. Sci. USA / PubMed:26183229
MethodsX-ray diffraction
Resolution1.549 - 3.01 Å
Structure data

PDB-4yvg:
Crystal Structure of H. influenzae TrmD in complex with AdoMet
Method: X-RAY DIFFRACTION / Resolution: 1.549 Å

PDB-4yvh:
Crystal Structure of H. influenzae TrmD in complex with sinefungin
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-4yvi:
Crystal Structure of H. influenzae TrmD in complex with sinefungin and tRNA
Method: X-RAY DIFFRACTION / Resolution: 3.01 Å

PDB-4yvj:
Crystal Structure of H. influenzae TrmD in complex with sinefungin and tRNA variant (G36U)
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

PDB-4yvk:
Crystal Structure of H. influenzae TrmD in complex with sinefungin and tRNA variant (G36C)
Method: X-RAY DIFFRACTION / Resolution: 3.002 Å

Chemicals

ChemComp-SAM:
S-ADENOSYLMETHIONINE

ChemComp-HOH:
WATER

ChemComp-SFG:
SINEFUNGIN

Source
  • haemophilus influenzae (strain atcc 51907 / dsm 11121 / kw20 / rd) (bacteria)
  • thermotoga maritima msb8 (bacteria)
KeywordsTRANSFERASE / MTase / SPOUT / TRANSFERASE/RNA / tRNA / TRANSFERASE-RNA complex

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