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Structure paper

TitleStructural analysis of the diadenylate cyclase reaction of DNA-integrity scanning protein A (DisA) and its inhibition by 3'-dATP.
Journal, issue, pagesBiochem. J., Vol. 469, Page 367-374, Year 2015
Publish dateMar 20, 2015 (structure data deposition date)
AuthorsMuller, M. / Deimling, T. / Hopfner, K.P. / Witte, G.
External linksBiochem. J. / PubMed:26014055
MethodsX-ray diffraction
Resolution2.25 - 2.55 Å
Structure data

PDB-4yvz:
Structure of Thermotoga maritima DisA in complex with 3'-dATP/Mn2+
Method: X-RAY DIFFRACTION / Resolution: 2.495 Å

PDB-4yxj:
Structure of Thermotoga maritima DisA in complex with ApCpp
Method: X-RAY DIFFRACTION / Resolution: 2.55 Å

PDB-4yxm:
Structure of Thermotoga maritima DisA D75N mutant with reaction product c-di-AMP
Method: X-RAY DIFFRACTION / Resolution: 2.25 Å

Chemicals

ChemComp-MN:
Unknown entry

ChemComp-3AT:
3'-DEOXYADENOSINE-5'-TRIPHOSPHATE

ChemComp-HOH:
WATER

ChemComp-APC:
DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER / AMP-CPP, energy-carrying molecule analogue*YM

ChemComp-2BA:
(2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

Source
  • thermotoga maritima (bacteria)
KeywordsDNA BINDING PROTEIN / c-di-AMP synthesis / DAC domain / Inhibitor / pre-reaction state / TRANSFERASE

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