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Structure paper

TitleHow a homolog of high-fidelity replicases conducts mutagenic DNA synthesis.
Journal, issue, pagesNat. Struct. Mol. Biol., Vol. 22, Page 298-303, Year 2015
Publish dateJan 27, 2015 (structure data deposition date)
AuthorsLee, Y.S. / Gao, Y. / Yang, W.
External linksNat. Struct. Mol. Biol. / PubMed:25775266
MethodsX-ray diffraction
Resolution2.95 - 3.301 Å
Structure data

PDB-4xvi:
Binary complex of human polymerase nu and DNA with the finger domain ajar
Method: X-RAY DIFFRACTION / Resolution: 3.1 Å

PDB-4xvk:
Binary complex of human polymerase nu and DNA with the finger domain closed
Method: X-RAY DIFFRACTION / Resolution: 2.95 Å

PDB-4xvl:
Binary complex of human polymerase nu and DNA with the finger domain open
Method: X-RAY DIFFRACTION / Resolution: 3.301 Å

PDB-4xvm:
Binary complex of human polymerase nu and DNA with the finger domain closed and thumb domain rotated out
Method: X-RAY DIFFRACTION / Resolution: 3.2 Å

Chemicals

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-NA:
Unknown entry

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
KeywordsTRANSFERASE/DNA / Pol Nu / Polymerase / error-prone DNA synthesis / TRANSFERASE-DNA complex

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