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TitleEvidence for the Formation of an Enamine Species during Aldol and Michael-type Addition Reactions Promiscuously Catalyzed by 4-Oxalocrotonate Tautomerase.
Journal, issue, pagesChembiochem, Vol. 16, Page 738-741, Year 2015
Publish dateNov 24, 2014 (structure data deposition date)
AuthorsPoddar, H. / Rahimi, M. / Geertsema, E.M. / Thunnissen, A.M. / Poelarends, G.J.
External linksChembiochem / PubMed:25728471
MethodsX-ray diffraction
Resolution1.7 - 1.944 Å
Structure data

PDB-4x19:
Crystal structure of native 4-OT from Pseudomonas putida mt-2 at 1.94 Angstrom
Method: X-RAY DIFFRACTION / Resolution: 1.944 Å

PDB-4x1c:
Crystal structure of 4-OT from Pseudomonas putida mt-2 with an enamine adduct on the N-terminal proline at 1.7 Angstrom resolution
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

Chemicals

ChemComp-NCO:
COBALT HEXAMMINE(III)

ChemComp-HOH:
WATER

Source
  • pseudomonas putida (bacteria)
KeywordsISOMERASE / 4-Oxalocrotonate tautomerase / beta-alpha-beta structural motif / tautomerase superfamily / enamine formation

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